University of Marburg, Germany, Dept. of Pharmacy

Research Assistant, Student Assistant Analytical Chemistry              1983-1987

Ph. D. (Dr.) Thesis in Pharmaceutical Chemistry                                1983-1987

University of Marburg, Germany, Dept. of Chemistry

Diploma in Chemistry                                                                       1976-1982

Editor-in-Chief of JPC (Journal of Planar Chromatography)                  since 2007

University of Applied Sciences Offenburg

Program Manager Process Engineering and Environmental Technologies

(Studiengangsleiter Verfahrens- und Umwelttechnik)                         2002-2010

Professor for Environmental Analysis and Recycling Technologies        since 1991

Ebulon GmbH (Schwitzerland)

Head of Analytical Research & Development                                   1990

Scheurich Pharmwerk Appenweier (Germany)

Head of the Analytical Unit                                                             1988-1990

B. Spangenberg, C. Pool, Ch. Weins

       Quantitative Thin-Layer Chromatography. A practical survey.

       Springer 2011

       B. Spangenberg,

       Chapter 46: Modern thin-layer chromatography in food analysis, in Yia H. Hui, Handbook of food science, technology and engineering, Band 1 2006

       „Location of Separated Zones by Use of Visualization Reagents, UV Absorbance on Layers Containing a Fluorescent Indicator, and Densitometry“ in

       T. Kowalska and J. Sherma „Preparative Layer Chromatography“, Taylor and Francis New York 2006

       B. Spangenberg

       “Derivatisation, detection (quantification) and identification of compounds on-line”, in “Thin-Layer Chromatography in Phytochemistry”, Editors: Joseph Sherma, Monika Waksmundzka-Hajnos, Taylor & Francis New York 2007

       B. Spangenberg

       Chapter 8: Quantitative detection of drugs by densitometry and video-scanning, in “Thin-Layer Chromatography in Drug Analysis”, Editors: Joseph Sherma, Lukasz Komsta, Monika Waksmundzka-Hajnos, Taylor & Francis New York 2012

/1/        Elschenbroich, B. Spangenberg, H. Mellinghoff
"1H-NMR-Koordinationsverschiebung und Ringstromschwächung bei Sandwichkomplexen, studiert am Beispiel Bis(6-[10]para-cyclophan)chrom(0)
Chem. Ber. 117 (1984), 3165-3168

/2/        Ch. Elschenbroich, J. Koch, J. Schneider B. Spangenberg, P. Schiess
Strained anellated arenes as -ligands: Synthesis and magnetic resonance properties of Bis(cyclobuta-6-benzene)chromium0,+1 and Bis[1,2:4,5]-Dicyclobuta-6-benzene)chromium0,+1
J. Organometall. Chem. 317 (1986), 41-54

/3/        W. Hanefeld und B. Spangenberg
"Umsetzung eines Iod-Ylids mit Methansulfonsäurechlorid"
Arch. Pharm. 320 (1987), 666-668

/4/        R. Allmann, W. Hanefeld, M. Krestel, B. Spangenberg
"Sulfene lassen sich umpolen"
Angew. Chem. 99 (1987), 1175
"A Sulfene Umpolung"
Angew. Chem. Int. Ed. Engl. 26 (1987) 1133

/5/        W. Hanefeld und B. Spangenberg
"Untersuchungen an β-Sultonen: Reaktionen in-situ-erzeugter Sulfene mit vicinalen
Tricarbonylverbindungen"
Arch. Pharm. 321 (1988), 85-88

/6/        W. Hanefeld, B. Spangenberg
"Reaktionen von Triethylammonium-1-(2-chlor-1,3-dioxo-2-indanyl)-chlormethansulfonat und -dichlormethansulfonat mit OH-, SH- und NH-Nucleophilen"
Arch. Pharm. 321 (1988) 253-257

/7/        W. Hanefeld, B. Spangenberg
"Ein inverser Aminangriff an Sulfen unter Bildung neuer Ammonio-sulfobetaine"
Chem. Ber. 121 (1988) 1147-1150

/8/        G. Blania, B. Spangenberg
"Allicin-Freisetzung aus getrocknetem Knoblauch (Allium sativum): Eine einfach durchzuführende HPTLC-Simultanbestimmung von Allicin und Ajoen in Knoblauchpulver und daraus hergestellten Fertigarzneimitteln"
Planta Medica 57 (1991) 371-375

/9/        C. Kraft, B. Spangenberg
"Eine einfache Bestimmung von Mineralölkohlenwasserstoffen"
Wasser Luft Boden  9 (1996), 33-34

/10/      B. Spangenberg
"A new proposal for a parameter-free integration software"
Fres. Z. Anal. Chem. 360 (1998), 148-151

/11/      B. Spangenberg, P. Hupfer, M. Jehle, J. Kress
"Dezentrale Kompostierung- sinnvolle Variante der Bioabfallverwertung?"
Müll und Abfall (1998), 70-74

/12/      B. Spangenberg
"Was hat Recycling mit Entropie zu tun?"
Müll und Abfall (2000), 502-504

/13/      B. Spangenberg, K.-F. Klein
"Fibre optical scanning with high resolution in thin-layer chromatography"
Journal of Chromatography A, 898 (2000) 265-269

/14/      B. Spangenberg, B. Ahrens, K.-F. Klein
"TLC-Analysis in Forensic Sciences Using a Diode-Array Detector"
Chromatographia 53 (2001) S438-S441

/15/      B. Spangenberg, K.-F. Klein
"New evaluation algorithm in Diode-Array Thin-Layer Chromatography"
Journal Planar Chromatography 14 (2001) 260-265

/16/      J. Stroka, B. Spangenberg, E. Anklam
"New Approaches in TLC Densitometry"
J. liq. chrom. & rel. technol. 25 (2002), 1497-1513

/17/      B. Spangenberg, P. Post, S. Ebel
"Fibre Optical Scanning in TLC by Use of a Diode-Array Detector - Linearization Models for Absorption and Fluorescence Evaluations"
Journal of Planar Chromatography 15 (2002) 88-93

/18/      B. Ahrens, D. Blankenhorn, B. Spangenberg
"Advanced fibre optical scanning in thin-layer chromatography for drug identification"
Journal of Chromatography B 772 (2002) 11-18

/19/      B. Spangenberg, K.-F. Klein, J. Mannhardt
"Proposals for Error-Reduction in Planar Chromatography"

            Journal of Planar Chromatography 15 (2002) 207-212

/20/      B. Spangenberg, K.Lorenz and St. Nasterlack
"Fluorescence Enhancement of Pyrene Measured by Thin-Layer Chromatography with Diode-Array Detection"

            Journal of Planar Chromatography 16 (2003) 328-334

/21/      J. Stroka, I. Arranz, J. Mac Court, B. Spangenberg,

            "A simple and reliable HPTLC method for the quantification of the intense sweetner Sucralose®"        

            J. liq. chrom. & rel. technol. 26 (2003) 2729 - 2739

/22/      B. Spangenberg, M. Weyandt-Spangenberg,

            "Quantitative Thin-Layer Chromatography using absorption and fluorescence Spectroscopy"

            Journal of Planar Chromatography 17 (2004) 164-168

/23/      J. Stroka, L. Dasko, B. Spangenberg, E. Anklam

            "Determination of the mycotoxin sterigmatocystin, by thin-layer chromatography and reagent-free derivatisation"

            J. liq. chrom. & rel. technol. 27 (2004) 2101 - 2111

/24/      B. Spangenberg, A. Seigel, J. Kempf and W. Weinmann,

            "Forensic Drug Analysis by Means of Diode-Array HPTLC using RF and UV Library Search "

            Journal of Planar Chromatography 18 (2005) 336-343

/25/      B. Spangenberg

            "Does the Kubelka-Munk Theory Describe TLC Evaluations Correctly? "

            Journal of Planar Chromatography 19 (2006) 332-341

/26/      B. Spangenberg, R. E. Kaiser,

            „The water content of stationary phases“,

            Journal of Planar Chromatography 20 (2007), 307 – 308

/27/      M. Bleichert, H. – S. Eckhardt, K.-F. Klein, B. Spangenberg

            „A simple and reliable method for quantification of glucosamine in nutritional

            supplements”,

            Journal of Planar Chromatography 21 (2008), 55 – 59

/28/      E. Jaryj, K. Lorenz, B. Spangenberg
"A simple method for the quantification of urethane in spirits"

            J. liq. chrom. & rel. technol. 31 (2008), 1-9

/29/      Melanie Broszat, Regina Brämer, and Bernd Spangenberg,

            „A new method for quantification of Melamine in milk by absorption Diode-Array Thin-Layer Chromatography”

            Journal of Planar Chromatography 21 (2008), 469 - 470

/30/      Meltem Göcer, Kathrin Hoferer, Jürgen Zipfel and Bernd Spangenberg,:

            „A new method for the quantification of Paraquat, Diquat, Difenzoquat, Mepiquat and Chloromequat in water by Thin-Layer Chromatography”

             Journal of Planar Chromatography 22 (2009), 59 - 63

/31/      Katharina Hiegel and Bernd Spangenberg

            „A new method for the quantification of Dequalinium-Cations in pharmaceutical samples by absorption and fluorescence diode array Thin-Layer Chromatography”

            Journal of Chromatography A 1216 (2009), 5052 - 5056

/32/      Jörg Stroka, Ivanka Doncheva, and Bernd Spangenberg

            „Determination of sucralose in soft drinks by HPTLC: Interlaboratory study”

            J. AOAC 92 (2009), 1153 - 1159

/33/      Melanie Broszat, Helena Ernst, Bernd Spangenberg
"A simple method for quantifying triazine herbicides using thin-layer chromatography and a CCD camera "

            J. liq. chrom. & rel. technol. 33 (2010),  948 - 956

/34/      Melanie Broszat, Christoph Welle, Monika Wojnowski, Helena Ernst, Bernd Spangenberg:

            „A versatile method for quantification of aflatoxins and ochratoxin A in dried figs ”

            Journal of Planar Chromatography 23 (2010), 193 -197

/35/      Andrea Seigel, Alexandra Schröck, Rebekka Hauser, Bernd Spangenberg
"Sensitive quantification of diclofenac and ibuprofen using thin-layer chromatography coupled with a vibrio fisheri bioluminescence assay "

            J. liq. chrom. & rel. technol. 34 (2011), 817 - 828

/36/      Bernd Spangenberg

            „ A new way of using chemiluminescence in thin-layer chromatography”

           Journal of Planar Chromatography 24 (2011), 357 – 359

/37/      Melanie Broszat, Helena Ernst, Bernd Spangenberg

            „Quantification of triazine herbicides using chloroplasts in conjuction with thin-layer chromotagoraphy”

            Environmental Biotechnology 7 (2011), 47 – 52

/38/      Barbara Milz, Idzaid Bin Idros, Bernd Spangenberg
"Limits of quantification of some neonicotinoid insecticides measured by thin-layer chromatography "

            J. liq. chrom. & rel. technol. 35 (2012), 1404 - 1414

/39/      Bernd Spangenberg
"Quantifying Quaternary Herbicides by Standard Addition Method”

           Journal of Planar Chromatography 25 (2012), 262 - 268

/1/        B. Spangenberg, W. Lieber, K.-F. Klein
"Neue Lichtleiter zur Mehrwellenlängendetektion in der Dünnschichtchromatographie"
InCom Tagungsband (1998), 180

/2/        M. Hübner, M. Meyer, K.-F. Klein, G. Hillrichs, M. Rüttling, M. Veidemanis, B. Spangenberg, J. Clarkin, G. Nelson
"Fibre-Optic Systems in the UV-Region"
SPIE-Proc., Vol. 3911 (BIOS`00), 303 - 312

/3/        K.-F. Klein, B. Spangenberg, H. Meyer, J. Mannhardt, J. Macomber
"New analytical applications with UV-fiber bundles"
SPIE-Proc., Vol. 4253 (BIOS`01), 168-175

/4/        B. Spangenberg, B. Ahrens, K.-F. Klein
"Diode-Array HPTLC in Forensic Sciences"
Beiträge zum XII Symposium der GTFCH in Mosbach 2001, 356-361

/5/        B. Spangenberg, K.-F.Klein: „New evaluation algorithm in diode-array thin-layer chromatography“. Proc. Of Planar Chromatography 2001 – New milestones in TLC, pp. 15-21 (Lillafüred/Ungarn, June 01) 

/6/        K. Klein, B. Spangenberg, B. Ahrens

             "Fluorescence in fiber optic HPTLC method",

            SPIE-Proc. BIOS'02, Vol. 4616, 212 - 219

/7/        B. Spangenberg, K.-F. Klein, J. Mannhardt
"High Performance Thin Layer Chromatography using Fiber-Optics and Diode-Array Detection", SPIE-Proc., Vol. 4625 (BIOS`02), 144 - 151

/8/        B. Spangenberg,: „Proposals for Error Reduction in Planar Chromatography “. Proc. Of Planar Chromatography 2002 – New milestones in TLC, pp. 93-105 (Budakalász/Ungarn, Juni 02) 

/9/        B. Spangenberg, B. Ahrens
"Quantitative Bestimmung von Clozapin im Serum mittels Dioden-Array Dünnschichtchromatographie "
Beiträge zum XIII Symposium der GTFCH in Mosbach 2003, 383-391

/10/      B. Spangenberg, K. Lorenz, S. Nasterlak: „Fluorescence Lifetime Measuremnts of Pyrene on HPTLC Plates “. Proc. Of Planar Chromatography 2003 – New milestones in TLC, pp. 3-11 (Budakalász /Ungarn, June 03) 

/11/      B. Spangenberg, M. Weyand-Spangenberg: „Fluorescence Evluation Using the Kubelka/Munk Formula“. Proc. Of Planar Chromatography 2004 – New milestones in TLC, pp. 125-136 (Visegrád/Ungarn, May 04) 

/12/      B. Spangenberg, A. Seigel, J. Kempf, W. Weinmann: „Forensic Drug Analysis by Means of Diode-Array HPTLC Using R_F and UV Library Search “. Proc. Of Planar Chromatography 2005 – New milestones in TLC, pp. 3-16 (Budakalász/Ungarn, May 05) 

/13/      K.-F. Klein, H.S. Eckhardt, B. Spangenberg: “Gas-sensing with hollow-core-waveguides“. NATO Summer School “Advanced Study Institute on Optical Waveguide Sensing and Imaging“ (Gatineau, Canada October 12-21, 2006)

/14/      H.S. Eckhardt, H. Dominick, K. T. V. Grattan, K. Graubner, K.-F. Klein, B. Spangenberg, and T. Sun:

            "Fiber-optic detection device for GC-UV",

            Proc. SPIE Vol. 6433, (2007),  paper 6433-12 (San Jose, USA, Jan.07)

/15/      M. Bleichert, H. S. Eckhardt, K.-F- Klein, B. Spangenberg, G. Hillrichs, J. Mannhardt

            “New components for fiber-optic thin-layer chromatography including fluorescence”,

            Proc. SPIE, Vol. 6433, paper 6433-14 (San Jose, USA, Jan.07)

/16/      H. S. Eckhardt, K.-F. Klein, B. Spangenberg, T. Sun and K. T. V. Grattan ,„Fibre-optic UV systems for gas and vapour analysis”,

             J. Phys.: Conf. Ser. 85 (2007), paper We12:20 (London, May 2007)

Invited Publications

/1/        B. Spangenberg, St. Stehle, Ch. Ströbele
"Quantitative DC mit einem Handscanner: Co2+-Bestimmung"
GIT 39 (1995), 461-464

/2/        B. Spangenberg, A. Seigel, R. Brämer, K.-F. Klein
"Dünnschichtchromatographie mittels Dioden-Array Detektion"
GIT Spezial Separation 1/2001, 37-39

/3/        B. Spangenberg, B. Ahrens, K.-F. Klein
"Spektren zur Substanzidentifikation in der Dioden-Array Dünnschichtchromatographie"
GIT Spezial Separation 1/2002, 22-24

/4/        B. Spangenberg und B. Ahrens
"Substanzidentifikation in der Dioden-Array Dünnschichtchromatographie"
GIT 6/2003, 658 - 660

/5/        Melanie Broszat, Regina Brämer, and Bernd Spangenberg,

·  „Was hat Recycling mit Entropie zu tun?“, Informationsreihe „Umwelt und Innovation“, IHK Südlicher Oberrhein, Lahr, Okt. 2000.

· “TLC-Analysis in forensic science by use of a diode-array TLC-Scanner”, 23rd International Symposium on Chromatography, Chester, UK, Okt. 2000.

· „Schnelle Wirkstoffanalyse in Blut und Urin mittels Dioden- Array Dünnschicht-chromatographie“, Uni Marburg, Aug. 2001.

· “New Evaluation Algorythm in Diode-Array Thin-Layer Chromatography”, Planar Chromatography 2001, Research Institut for Medical Plants, Budakalász, Ungarn, Juni 2001.

· “Diodenarraygekoppelte Dünnschichtchromatographie”, Universität Münster, 15.01.2002.

· „New Aspects in Diode- Array TLC” Joint Research Centre Ispra, Italien, 26. März 2002.

· „Proposals for Error Reduction in Planar Chromatography”, International Society for Planar Chromatographie, Ungarn, 13. Mai 2002.

· “Fluorescence Lifetime Measurements of Pyrene on HPTLC-Plates”. Planar Chromatography 2003, Budapest, Ungarn, 21.-23. Juni 2003.

· “What can Diode-Array Planar Chromatography Perform?”, 5th Balaton Symposium, Siófok, Ungarn, 3.-5.09.2003.

· „Diodearray-Detektion in der Dünnschichtchromatographie“, GDCh-Kurs, Isny, 4.-6.11.2003.

· „Diodearray-Detection in Thin-Layer Chromatography“, Joint Research Centre of the European Commission, Institute for Reference Materials and Measurements (IRMM), 01.11.2002 bis 31.01. 2003.

·  “What can Diode-Array Planar Chromatography Perform?”, TLC-Konferenz, Lublin-

Kazimierz Dolny, Polen, Juni 2004

·  „Was leistet die Dioden-Array DC?“, Universität Tübingen, Pharmazeutische Fakultät, April 2004

·  „Fluorescence Evaluation Using the Kubelka/Munk Formula”, Visegrad, Ungarn, Mai 2004

· “Forensic Drug Analysis by Means of Diode-Array HPTLC using RF und UV Library Search”, Siofok, Ungarn, 29 Mai 2005.

· „Möglichkeiten und Grenzen der Diodenarray Planarchromatographie“, Isny, 23. Sept. 2005.

· „Neue Ergebnisse in der Bioanalytik“, Biovalley Stammtisch Offenburg, 27. Okt. 2005.

· “A new way of fluorescence scanning in TLC“, International Symposium for Thin-Layer Chromatography, 10.10-2006, Berlin

· “A simple and reliable method to quantify glucosamine in various nutritional supplements” The 7th Balaton Symposium,in Siófok, Hungary, on September 5–7, 2007

· “A new method for the quantification of Quats by TLC”, International Symposium for Thin-Layer Chromatography, 11. – 13. June 2008, Helsinki

· „Fluorescence spectro-scopy for HPTLC separations in phyto-chemical analysis“, 6^Th InternationalSymposium on Chromatography of Natural Products, Lublin, Poland, 15^Th – 18, June 2008

· „Aflatoxins in Figs. An Analytical Problem?”, 8th Balaton Symposium on High Performance Separation Methods, September 2-4, 2009, Siofok, Hungary

· „ A Versatile Method for Quantifying Aflatoxins and Ochratoxin A Present in Dried Figs”,

Analytica Conference  München, 24.3.2010

·„Wie lässt sich die Bestimmungsgrenze in der DC-Analyse verbessern?“, Universitity

 Hohenheim, Fachbereich Lebensmittelanalytik, 19. Mai 2011, Stuttgart-Hohenheim

· „ A New Approach for a Multidimensional TLC Evaluation”, 17th International Symposium

 on Separation Sciences (17-ISSS), September 5-9, 2011, Cluj-Napoca, Romania